Question 1

A biopsy is:
a. Removal of a biological fluid
b. Removal of an organ
c. Excision of a small tissue sample Correct
d. A postmortem examination
e. Always done in a hospital

Correct Answer

c. Excision of a small tissue sample
Rationale: A biopsy involves removing a small sample of tissue for diagnostic examination under a microscope.

Question 2

An autopsy is:
a. Any surgical procedure
b. Another name for an operation
c. An excision
d. An incision
e. Removal of tissue from a dead body Correct

Correct Answer

e. Removal of tissue from a dead body
Rationale: An autopsy is a postmortem examination to study the body and determine the cause of death or disease.

Question 3

Tissue for STAT processing is:
a. Put into fixative immediately
b. Kept in the freezer
c. Wrapped in saline-soaked gauze until gross specimen treatment Correct
d. Sealed in an air-tight container
e. Immersed in water and kept in the fridge

Correct Answer

c. Wrapped in saline-soaked gauze until gross specimen treatment
Rationale: For urgent processing, tissue must remain moist and fresh by wrapping it in saline-soaked gauze to prevent desiccation or degradation.

Question 4

Identify the false statement regarding a fixative:
a. It prevents cell autolysis and putrefaction
b. It is the first step in routine histology processing
c. It takes 15-20 minutes Correct
d. It acts as a mordant to make staining more effective
e. It allows tissue to be examined in a life-like manner

Correct Answer

c. It takes 15-20 minutes
Rationale: Fixation typically takes hours, not minutes, to fully preserve tissue structure and prevent decay.

Question 5

Which reagent is routinely used to fix tissue?
a. 10% Buffered Formalin Correct
b. Zenker’s Fluid
c. 95% Ethanol
d. Acid Alcohol
e. Bouin’s Fluid

Correct Answer

a. 10% Buffered Formalin
Rationale: 10% Buffered Formalin is the most commonly used fixative in histology for preserving tissue.

Question 6

Decalcification would be done:
a. Only if time permits
b. After embedding
c. Right before staining
d. Before fixation
e. After fixation

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Question 7

What is not used to decalcify tissue?
a. Formic acid
b. Saline
c. Nitric acid
d. Electrophoresis
e. EDTA

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Question 8

A good decalcifying reagent should:
a. Minimize damage to tissue
b. Work in a reasonable amount of time
c. Not interfere with staining procedures
d. Completely remove calcium
e. All of these are correct

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Question 9

EDTA is a “chelating agent”. This means it attaches or binds:
a. Prothrombin
b. Sodium ions
c. Hydrogen ions
d. Calcium ions
e. Potassium ions

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Question 10

Which reagent(s) is harmful to the lab worker?
a. Formalin
b. Xylene
c. Zenker’s fluid
d. Bouin’s fluid
e. All the above

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Question 11

When tissues are embedded, the embedding medium replaces:
a. Formalin
b. Paraffin
c. Dehydrating agents
d. Clearing agents
e. Hydrating agents

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Question 12

A mold for embedding can be made of:
a. Wood
b. Cardboard
c. Wax
d. Plastic or metal
e. Rubber

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Question 13

The sectioning of tissue produces:
a. Ribbons
b. Strips
c. Panels
d. Plates
e. Layers

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Question 14

Which substance is not used for embedding?
a. Wax
b. Resin
c. Agar
d. Plastic
e. EDTA

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Question 15

During sectioning, the ideal thickness of a paraffin-embedded tissue slice is:
a. 1 µm
b. 5 µm
c. 10 µm
d. 20 µm
e. 50 µm

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Question 16

Tissue embedding is done to:
a. Preserve the tissue in a life-like state
b. Allow thin sections to be cut
c. Remove calcium
d. Prevent contamination
e. Hydrate the tissue

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Question 17

Which instrument is used for sectioning tissues?
a. Microtome
b. Cryostat
c. Thermometer
d. Homogenizer
e. Ultracentrifuge

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Question 18

Frozen sections are typically cut using:
a. A cryostat
b. A microtome
c. A freezing microtome
d. Both a and c
e. A centrifuge

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Question 19

Which is not a clearing agent?
a. Xylene
b. Benzene
c. Chloroform
d. Formalin
e. Toluene

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Question 20

Dehydration of tissue is performed using:
a. Acid alcohol
b. Formalin
c. Ethanol
d. Distilled water
e. Sodium chloride

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Question 21

For staining purposes, the section is usually mounted on:
a. Metal slides
b. Plastic covers
c. Glass slides
d. Agar plates
e. Paraffin blocks

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Question 22

What is not a part of the staining process?
a. Deparaffinization
b. Hydration
c. Decalcification
d. Differentiation
e. Staining

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Question 23

The hematoxylin and eosin stain:
a. Is used for nuclear staining only
b. Is used for cytoplasmic staining only
c. Is used for both nuclear and cytoplasmic staining
d. Is a special stain
e. None of the above

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Question 24

Which of the following is a basic dye?
a. Eosin
b. Hematoxylin
c. India ink
d. Congo red
e. Acid fuchsin

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Question 26

Which stain is used to detect glycogen in tissues?
a. Alcian blue
b. PAS stain
c. Hematoxylin
d. Oil Red O
e. Giemsa stain

Question 27

The purpose of differentiation in staining is to:
a. Increase contrast between different tissue components
b. Remove excess stain from tissues
c. Hydrate the tissues
d. Embed the tissues
e. Fix the tissues

Question 28

Which stain is used for lipid detection?
a. Sudan III
b. Hematoxylin
c. Eosin
d. PAS
e. Alcian blue

Question 29

Which of the following stains is used for bacteria?
a. Giemsa stain
b. Gram stain
c. PAS stain
d. Trichrome stain
e. Alcian blue

Question 30

The Ziehl-Neelsen stain is used to identify:
a. Gram-positive bacteria
b. Acid-fast bacteria
c. Fungi
d. Glycogen
e. Lipids

Question 31

A counterstain is used to:
a. Remove the primary stain
b. Provide contrast to the primary stain
c. Enhance the embedding process
d. Fix the tissue
e. Decalcify the tissue

Question 32

Which dye combination is used in Romanowsky stains?
a. Hematoxylin and eosin
b. Methylene blue and eosin
c. Methylene blue and azure
d. Sudan III and PAS
e. Congo red and eosin

Question 33

Which of the following is an example of a silver stain?
a. PAS
b. Giemsa
c. Gomori’s stain
d. Alcian blue
e. Masson’s trichrome

Question 34

Which of the following is a fluorescent dye?
a. Fluorescein
b. PAS
c. Alcian blue
d. Eosin
e. Sudan IV

Question 35

What is the purpose of mounting a tissue section?
a. To fix the tissue
b. To enhance the staining process
c. To prepare the tissue for microscopic observation
d. To dehydrate the tissue
e. To remove clearing agents

Question 36

Which type of slide mounting medium is most commonly used?
a. Water-based medium
b. Resin-based medium
c. Oil-based medium
d. Agar-based medium
e. Gel-based medium

Question 37

Which staining method is used to demonstrate fungi in tissue sections?
a. PAS stain
b. Alcian blue
c. Giemsa stain
d. Masson’s trichrome
e. Hematoxylin and eosin

Question 38

What is the primary purpose of clearing in tissue processing?
a. To dehydrate the tissue
b. To infiltrate the tissue with paraffin
c. To make tissue transparent
d. To fix the tissue
e. To stain the tissue

Question 39

Which is a decalcification agent?
a. Ethanol
b. Xylene
c. EDTA
d. Formalin
e. Paraffin

Question 40

What is the best method for preserving lipids in tissue?
a. Freezing
b. Embedding in paraffin
c. Fixation with formalin
d. Staining with PAS
e. Dehydration

(Solution) Anatomic Pathology Quiz

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